MilliporeSigma™ Amicon™ Bioseparations Stirred Cells†

Description

Includes

Connection for 0.25 in. dia. (6.4mm) pressure tubing; max. operating pressure, 75psi (5.3 bar).

Requires

Magnetic stirrer required for operation.

Concentrate, desalt, and purify aqueous biological solutions

Process from 3mL up to 400mL of biological solutions using either concentration or diafiltration operating modes.

• Sturdy construction; autoclavable Consist of clear pressurized vessel with integral magnetic stirring bar, nylon cap and tube fitting assembly, polysulfone body and membrane holder, acetal/polysulfone stirrer, silicone O-rings, and black aluminum stand

Concentration Mode

• Gas pressure is applied directly to ultrafiltration cell Solutes above membrane molecular weight cutoff are retained in cell, while water and solutes below cutoff pass into filtrate

Diafiltration Mode

Ultrafiltration cell is connected to auxiliary reservoir containing diafiltrate solution; reservoir is then gas pressurized Fluid volume and macrosolute concentration remain constant as filtrate is replaced by diafiltrate solution Provides a simple means for rapid microsolute exchange
• Typically used as a substitute for dialysis

Applications:

Perform concentration, desalting, recovery or exclusion; Recover monoclonal antibodies and growth factors from tissue culture supernatants; Concentration and buffer exchange of chromatography fractions

Order Info

Use with Millipore Ultrafiltration Membranes.

Specifications

• Autoclavable: Autoclavable
• Concentration: 0.05 to 10g%
• Material: Nylon cap and tube fitting assembly, Polysulfone cylinder and membrane holder, Acetal and Polysulfone stirring assembly, Silicone Rubber O-rings, Polyethelene pressure tube, Tygon tubing filtrate tube, Anodized Aluminum or Nylon retaining stand
• Filtration Area: 28.7 cm²
• For Use With (Application): Protein purification, protein concentration, protein desalting
• Includes: Clear pressurized vessel with integral magnetic stirring bar, nylon cap and tube fitting assembly, polysulfone body and membrane holder, acetal/polysulfone stirrer, silicone O-rings and black aluminum stand, connections for 0.25 in. dia. pressure tubing
• Model: 8200
• Volume (English): 0.16 to 6.7 oz.
• Volume (Metric): 5 to 200 mL
• Volume (Metric) Hold Up: 1200 μL
• Dimensions (L x W x H): 3.5 x 3.5 x 5.03 in. (9 x 9 x 12.8cm)
• Length (English): 3.5 in.
• Length (Metric): 9 cm
• Width (English): 3.5 in.
• Width (Metric): 9 cm
• Height (English): 5.03 in.
• Height (Metric): 12.8 cm
• Diameter (English): 2.5 in.
• Diameter (Metric): 6.35 cm