MilliporeSigma™ AMPK (α1/β1/γ1), HisTag™, Human, Recombinant Protein, S. frugiperda

Catalog No.	Quantity
17-153-65UG	5 μg

Catalog No. 17-153-65UG Supplier MilliporeSigma™ Supplier No. 1715365UG

Description

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His·Tag™ sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening.

Specifications

• Formulation: In 300mM NaCl, 150mM Imidazole, 50mM Sodium Phosphate, 0.2mM DTT, 0.1mM PMSF, 25% glycerol, pH 7.0.
• Purification Method: SDS-PAGE
• Quantity: 5 μg
• Source: S. frugiperda
• Immunogen: AMPK (α1/β1/γ1)
• Storage Requirements: Following initial thaw, aliquot and freeze (−70°C).
• Regulatory Status: RUO
• Conjugate: His-Tag
• Species: Human
• Recombinant: Recombinant
• Form: Liquid
• Purity or Quality Grade: ≥95%