MilliporeSigma™ Upstate™ CHD1, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 1710533

Catalog No. 17-105-33MI

Description

Specifically detects CHD1 Clone: in Human samples, and it is validated for ChIP, Western Blotting

Chromodomain-helicase-DNA-binding protein 1 (CHD1) is a multidomain chromatin-remodelling enzyme, which belongs to the SNF2/RAD54 helicase family. It contains an SWI/SNF-like helicase/ATPase domain, a DNA-binding domain, and two chromodomains. CHD1 is multifunctional protein, which has an important role in gene regulation. It interacts with various modified histones, such as H3K4Me2 and H3K4Me3, and maintains the open chromatin structure required for transcription. It also interacts with, or plays a role in the assembly of, several transcription-related complexes, including the histone acetylation complex SAGA, and the FACT and PAF complexes. Previous studies have suggested that CHD1 maintains the pluripotent state of embryonic stem cells, and is required for the reprogramming of somatic cells to the pluripotent state. CHD1 may play a role in the development of prostate cancer.

Specifications

• Antigen: CHD1
• Classification: Polyclonal
• Formulation: Anti-CHD1 (Rabbit Polyclonal). One vial containing 7.0μg purified rabbit polyclonal in 50μL buffer of Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide, before the addition of 30% glycerol (0.14mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSMC6. One vial containing 75μL of 5μM of each primer specific for human proteasome 26S subunit 6 (chr14:53174161+53174274 , hg19 build). Primer location selection based on ENCODE browser data at UCSC, see also reference 1. FOR: TCC GGC CCT GAG CTT GT; REV: CCT CCT CTA CTT CTT TTT CAT TTT CAC
• Gene Symbols: Chd1, Chd-1
• Immunogen: Recombinant protein corresponding to human CHD1 (a.a. 1660-1710).
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes CHD1 in regions between a.a. 1660 to 1710.
• Target Species: Human
• Form: Purified

• Applications: ChIP Assay, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: O14646
• Host Species: Rabbit
• Quantity: 10 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_001261
• Includes: This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only