MilliporeSigma™ Upstate™ CHD1, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-105-33MI	10 Assays	Unconjugated

Catalog No. 17-105-33MI Supplier MilliporeSigma™ Supplier No. 1710533

Description

Rabbit Polyclonal Antibody

Specifically detects CHD1 Clone: in Human samples, and it is validated for ChIP, Western Blotting

Chromodomain-helicase-DNA-binding protein 1 (CHD1) is a multidomain chromatin-remodelling enzyme, which belongs to the SNF2/RAD54 helicase family. It contains an SWI/SNF-like helicase/ATPase domain, a DNA-binding domain, and two chromodomains. CHD1 is multifunctional protein, which has an important role in gene regulation. It interacts with various modified histones, such as H3K4Me2 and H3K4Me3, and maintains the open chromatin structure required for transcription. It also interacts with, or plays a role in the assembly of, several transcription-related complexes, including the histone acetylation complex SAGA, and the FACT and PAF complexes. Previous studies have suggested that CHD1 maintains the pluripotent state of embryonic stem cells, and is required for the reprogramming of somatic cells to the pluripotent state. CHD1 may play a role in the development of prostate cancer.

Specifications

• Antigen: CHD1
• Applications: ChIP Assay, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-CHD1 (Rabbit Polyclonal). One vial containing 7.0μg purified rabbit polyclonal in 50μL buffer of Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide, before the addition of 30% glycerol (0.14mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PSMC6. One vial containing 75μL of 5μM of each primer specific for human proteasome 26S subunit 6 (chr14:53174161+53174274 , hg19 build). Primer location selection based on ENCODE browser data at UCSC, see also reference 1. FOR: TCC GGC CCT GAG CTT GT; REV: CCT CCT CTA CTT CTT TTT CAT TTT CAC
• Gene Accession No.: O14646
• Gene Symbols: Chd1, Chd-1
• Host Species: Rabbit
• Immunogen: Recombinant protein corresponding to human CHD1 (a.a. 1660-1710).
• Quantity: 10 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001261
• Test Specificity: Recognizes CHD1 in regions between a.a. 1660 to 1710.
• Includes: This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
• Target Species: Human
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified

For Research Use Only