MilliporeSigma™ Upstate™ ChIPAb+™ Phospho-Histone H3 (Thr3)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-101-41MI	25 Assays	Unconjugated

Catalog No. 17-101-41MI Supplier MilliporeSigma™ Supplier No. 1710141

Description

Rabbit Monoclonal Antibody

Specifically detects ChIPAb+™ Phospho-Histone H3 (Thr3)α Clone: in Human samples, and it is validated for Chromatin Immunoprecipitation, Dot Blot, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: ChIPAb+™ Phospho-Histone H3 (Thr3)α
• Applications: ChIP Assay, Dot Blot, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-Phospho-Histone H3 (Thr3) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: Q16695
• Gene Symbols: HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3.4, H3T, H3/g, H3/t
• Host Species: Rabbit
• Immunogen: Peptide containing the sequence [RT_trimKQ] in which lysine 4 is trimethylated on human histone H3
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003493
• Test Specificity: Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
• Includes: This ChIPAb+ Phospho-Histone H3 (Thr3) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Culture Supernatant

For Research Use Only