MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys27)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-101-08MI	25 Assays	Unconjugated

Catalog No. 17-101-08MI Supplier MilliporeSigma™ Supplier No. 1710108

Description

Rabbit Monoclonal Antibody

Specifically detects Dimethyl-Histone H3 (Lys27)α Clone: in Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the histone code hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone H3 is methylated at Lys27 by EZH2, and and overexpression of EZH2 has been associated with both breast and prostate cancers. Methylation of H3K27 is involved in X chroosome inactivation, imprinting, circadian rhythms, and stem cell maintenance. H3K27me2 is a marker of classical heterochromatin.

Specifications

• Antigen: Dimethyl-Histone H3 (Lys27)α
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-Dimethyl-Histone H3 (Lys27) (rabbit monoclonal). One vial containing 50μL of cultured supernantant in 0.05% sodium azide. Negative Control Supernatant. One vial containing 100μL of rabbit cultured supernatant in 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
• Gene Accession No.: Q71DI3
• Gene Symbols: H3, H3.2, H3/M, H3/m, H3/o, H3F2, H3FM, MGC9629, OTTHUMP00000014041
• Host Species: Rabbit
• Immunogen: Peptide containing the sequence (ARme2KSA) in which me2 corresponds to dimethyl lysine at residue 27 of human histone H3.
• Purification Method: Unpurified supernatant
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484.1
• Test Specificity: This antibody recognizes Histone H3 dimethylated on Lys27.
• Includes: This ChIPAb+ Dimethyl-Histone H3 (Lys27) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Vertebrates
• Content And Storage: −20°C for one year from date of shipment
• Form: Culture Supernatant
• Isotype: IgG

For Research Use Only