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MilliporeSigma™ Upstate™ Dimethyl-Histone H3 (Lys9), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No. 17648MI Shop All MilliporeSigma Products
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Quantity:
25 Assays
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
17-648-MI 25 Assays Unconjugated
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Catalog No. 17-648-MI Supplier MilliporeSigma™ Supplier No. 17648
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Description

Rabbit Polyclonal Antibody

Specifically detects Dimethyl-Histone H3 (Lys9) Clone: in Human, Mammalia, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Immunoprecipitation, Western Blotting

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Dimethyl-Histone H3 (Lys9)
Applications ChIP Assay, Immunoprecipitation, Western Blot
Classification Polyclonal
Conjugate Unconjugated
Formulation Dimethyl-Histone H3 (Lys9) (rabbit polyclonal serum). One vial containing 100μL of antiserum containing 0.05% sodium azide.
Normal Rabbit Serum. One vial containing 100μL antiserum containing 0.05% sodium azide.
ChIP Primers, β-Globin. One vial containing 75μL of 5μM of each primer specific for human β-globin. FOR: AGG ACA GGT ACG GCT GTC ATC REV: TTT ATG CCC AGC CCT GGC TC
Gene Accession No. Q66I33
Gene Symbols H3F3A, H3F3B, H3F3
Host Species Rabbit
Immunogen The dimethyl-histone H3 (Lys9) rabbit serum is made against a KLH-conjugated, branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human histone H3
Quantity 25 Assays
Regulatory Status RUO
Research Discipline Epigenetics and Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NM_003493
Test Specificity Dimethyl-Histone H3 (Lys9)
Includes Dimethyl-Histone H3 (Lys9) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K9Me2.
Target Species Human, Mammalia, Mouse
Content And Storage −20°C from date of receipt
Form Serum
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For Research Use Only

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