MilliporeSigma™ Upstate™ Dimethyl-Histone H4 (Arg3) Symmetric, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-50MI	25 Assays	Unconjugated

Catalog No. 17-102-50MI Supplier MilliporeSigma™ Supplier No. 1710250

Description

Rabbit Polyclonal Antibody

Specifically detects Dimethyl-Histone H4 (Arg3) Symmetric Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Dot Blot, Western Blotting

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Arginine modifications of histones proteins are mediated by two classes of protein arginine methyltransferases (PRMTs), class I and II. Class I PRMTs place two methyl groups on the one nitrogen atom of the arginine guanidino group, resulting in an asymmetric modification. In contrast class II PRMTs place two methyl groups on two nitrogen atoms of the guanidino group producing a symmetric dimethylation. Although there are exceptions, asymmetric modifications are typically associated with transcription activation while symmetric modifications are most commonly associated with transcriptional repression.

Specifications

• Antigen: Dimethyl-Histone H4 (Arg3) Symmetric
• Applications: ChIP Assay, Dot Blot, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Dimethyl-Histone H4 (Arg3) Symmetric (rabbit polyclonal). One vial containing 17.5μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7μg/μL. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
• Gene Accession No.: P62805
• Gene Symbols: HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to human Histone H4 when symmetrically dimethylated at Arg3.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001029249
• Test Specificity: This antibody recognizes Histone H4 symmetrically dimethylated at Arg3.
• Includes: This ChIPAb+ Dimethyl-Histone H4 (Arg3) Symmetric -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only