MilliporeSigma™ Upstate™ E2F-1α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-100-61MI	25 Assays	Unconjugated

Catalog No. 17-100-61MI Supplier MilliporeSigma™ Supplier No. 1710061

Description

Mouse Monoclonal Antibody

Specifically detects E2F-1α Clone: in Human, Xenopus samples, and it is validated for ChIP, ChIP-seq, Immunoprecipitation

E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Specifications

• Antigen: E2F-1α
• Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Immunoprecipitation
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-E2F-1 (mouse monoclonal). One vial containing 25μg of protein G purified monoclonal mixed IgG in 25μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. Nornal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, CDC2 promoter. One vial containing 75μL of 5μM each primer specific for human CDC2 promoter. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT
• Gene Accession No.: Q01094
• Gene Symbols: E2F1, RBAP-1, E2F-1, RBP3, RBBP3, RBBP-3, PBR3
• Host Species: Mouse
• Immunogen: GST fusion protein corresponding to full-length human E2F-1.
• Purification Method: Protein G purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005216
• Test Specificity: The epitopes have been mapped to amino acids 1 to 89 and to amino acids 342 to 386.
• Includes: This ChIPAb+ E2F-1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Xenopus species
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG

For Research Use Only