MilliporeSigma™ Upstate™ E2F-3α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 1710062

Catalog No. 17-100-62MI

Description

Specifically detects E2F-3α Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Western Blotting

E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.

Specifications

• Antigen: E2F-3α
• Classification: Monoclonal
• Formulation: Anti-E2F-3 (mouse monoclonal), . One vial containing 25μg of protein A purified mouse IgG2a in ∽180μL of storage buffer (10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before addition of 30% glycerol). Normal Mouse IgG. One vial containing 25μg purified IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, human CDC2 coding region, . One vial containing 75μL of 5μM each primer specific for human CDC2 coding region. FOR: CGC CCT TTC CTC TTT CTT TC; REV: ATC GGG TAG CCC GTA GAC TT
• Gene Symbols: E2F3, KIAA0075, E2F-3, MGC104598, DKFZp686C18211
• Immunogen: Recombinant full-length human E2F-3 protein
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_001940.1
• Includes: This ChIPAb+ E2F-3 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Isotype: IgG2a

• Applications: ChIP Assay, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: O00716
• Host Species: Mouse
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: E2F-3
• Target Species: Human, Mouse, Rat
• Form: Purified

For Research Use Only