MilliporeSigma™ ESGRO™-2i Selective Medium Kit

Description

provided with a selective GSK3β and Mek 1/2 inhibitors to enhance viability of mouse embryonic stem and iPS cells in the absence of serum and feeder cells. Supports a naive/ground pluripotent state.

Two stages of mouse pluripotency have been stabilized to date through in vitro culture. ‘Naive’ represents the earliest stage, which can be enriched for a purer population, termed ‘ground state’. Naive most closely approximates embryonic epiblast in the preimplantation blastocyst. The primed state most closely approximates in vivo immediate postimplantation epiblast. There are clear differences between these stages in culture, expression, epigenetics (including appropriate X-inactivation) and developmental competence. The fact that naive mESCs can generate whole animals through tetraploid complementation while primed mESCs cannot, is a particularly compelling reason for heightened expectations from naive hESCs.

Specifications

• For Use With (Application): Stem Cell Culture
• Quantity: 200 mL
• Content And Storage: Store in dark at -20° C