MilliporeSigma™ Upstate™ EZH2, clone AC22α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-662-MI	25 Assays	Unconjugated

Catalog No. 17-662-MI Supplier MilliporeSigma™ Supplier No. 17662

Description

Mouse Monoclonal Antibody

Specifically detects EZH2, clone AC22α Clone: in Human, Mouse, Rat, Vertebrate samples, and it is validated for ChIP, Western Blotting

EZH2 is a polycomb group (PcG) protein that is a catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

Specifications

• Antigen: EZH2, clone AC22α
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials, each containing 25μg purified mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers MYT-1 promoter Region. One vial containing 75μL of 5μM of each primer specific for the promoter region of human MYT-1. FOR: ACA AAG GCA GAT ACC CAA CG; REV: GCA GTT TCA AAA AGC CAT CC
• Gene Accession No.: Q15910
• Gene Symbols: EZH2; KMT6
• Host Species: Mouse
• Immunogen: EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function, Apoptosis and Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_004447.2
• Test Specificity: Recognizes EZH2, MW: ∽85-100kDa.
• Includes: EZH2, clone AC22, ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of EZH2.
• Target Species: Human, Mouse, Rat, Vertebrates
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG2a

For Research Use Only