MilliporeSigma™ Upstate™ FOXA1, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-67MI	25 Assays	Unconjugated

Catalog No. 17-102-67MI Supplier MilliporeSigma™ Supplier No. 1710267

Description

Rabbit Polyclonal Antibody

Specifically detects FOXA1 Clone: in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Immunocytochemistry, Western Blotting

HNF-3A, Hepatocyte nuclear factor 3-alpha (also called FOXA1) is member of the Forkhead family of winged-helix transcription factors. This protein family has around 50 known transcription factors and play an important role in the regulation of cell differentiation, organogenesis, and gene expression. High levels of expression of HNF-3A has been observed in tumors arising from both the prostate and breast. Here it is believed this protein interacts with the androgen receptor in prostate tumors and the estrogen receptor α in breast tumors. In the case of ERα, HNF-3A interacts with cis-regulatory regions in heterochromatin and enhances the interaction of ERα with chromatin.

Specifications

• Antigen: FOXA1
• Applications: ChIP Assay, Immunocytochemistry, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-FOXA1 (Rabbit Polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.35mg/mL. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75μL of 5μM of each primer specific for Mouse Hnf4α enhancer. FOR: TTC CAG CTG CCT TTA TCT CCC TGT; REV: TCT CCA CAC ATG TCC AGC AGC CT
• Gene Accession No.: P55317
• Gene Symbols: FOXA1, HNF-3-alpha, HNF-3A, HNF3A, TCF3A, MGC33105
• Host Species: Rabbit
• Immunogen: Recombinant protein corresponding to the entire protein of human FOXA1
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_004487
• Test Specificity: This antibody recognizes HNF-3A/FOXA1.
• Includes: This ChIPAb+ FOXA1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only