MilliporeSigma™ Upstate™ FOXA2α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-58MI	25 Assays	Unconjugated

Catalog No. 17-102-58MI Supplier MilliporeSigma™ Supplier No. 1710258

Description

Mouse Monoclonal Antibody

Specifically detects FOXA2α Clone: in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Immunocytochemistry, Western Blotting

Also known as hepatocyle nuclear factor 3-beta (HNF-3B), Forkhead box protein A2 (FOXA2) belongs to the forkhead class of DNA-binding proteins. This family of transcription factors influences gene expression in endodermally-derived tissues such as lung, liver, stomach, pancreas, and intestine. Foxa2 has also been detected in ectodermally-derived tissues such as kidney, uterus and vagina, and seminal and coagulating glands during development. Several studies show that FOXA proteins help facilitiate the binding of several nuclear receptors to their respective targets in a context-dependent manner, and are thereby implicated in various functions including cellular maintenance, differentiation, metabolism, and organogenesis. Aberrant function of FOXA proteins are attributed to diseases states such as diabetes and Parkinson's disease.

Specifications

• Antigen: FOXA2α
• Applications: ChIP Assay, Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-FOXA2 (mouse monoclonal),. One vial containing 100μL of purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) and 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.175mg/mL. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75μL of 5μM of each primer specific for Mouse Hnf4α enhancer. FOR: TTC CAG CTG CCT TTA TCT CCC TGT; REV: TCT CCA CAC ATG TCC AGC AGC CT
• Gene Accession No.: Q9Y261
• Gene Symbols: FOXA2, HNF3B, TCF3B
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to the human FOXA2.
• Purification Method: Protein G
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_068556
• Includes: This ChIPAb+ FOXA2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only