MilliporeSigma™ Upstate™ HDAC2α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-37MI	25 Assays	Unconjugated

Catalog No. 17-102-37MI Supplier MilliporeSigma™ Supplier No. 1710237

Description

Mouse Monoclonal Antibody

Specifically detects HDAC2α Clone: in Bovine, Hamster, Human, Mouse, Rat samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Specifications

• Antigen: HDAC2α
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-HDAC2 (mouse monoclonal). One vial containing 50μg of purified mouse monoclonal IgG in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Concentration: 0.94mg/mL. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, VWF promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human von Willebrand factor. FOR: GCT GAG AGC ATG GCC TAG GGT GGT GGG CGG CAC; REV: CCC CTG CAA ATG AGG GCT GCG GCT ATC TCC AAG
• Gene Accession No.: Q92769
• Gene Symbols: HDAC2, HD2, YAF1, RPD3
• Host Species: Mouse
• Immunogen: KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to human HDAC2 at the C-terminus.
• Purification Method: Protein G purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001518
• Test Specificity: This antibody recognizes HDAC2 at the C-terminus.
• Includes: This ChIPAb+ HDAC2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Bovine, Hamster, Human, Mouse, Rat
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG1

For Research Use Only