MilliporeSigma™ Upstate™ HDAC3 Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-38MI	25 Assays	Unconjugated

Catalog No. 17-102-38MI Supplier MilliporeSigma™ Supplier No. 1710238

Description

Mouse Monoclonal Antibody

Specifically detects HDAC3 Clone: in Bovine, Hamster, Human, Mouse samples, and it is validated for ChIP, Western Blotting

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene.

Specifications

• Antigen: HDAC3
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-HDAC3 (mouse monoclonal). One vial containing 100μg of protein G purified monoclonal in buffer containing 0.02M phosphate buffer (pH 7.6), 0.25M NaCl, 0.1% sodium azide and 30% glycerol. Concentration: 1mg/mL. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, p21. One vial containing 75μL of each primer (5μM) specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. FOR: CCC ACA GCA GAG GAG AAA GAA; REV: CTG GAA ATC TCT GCC CAG ACA
• Gene Accession No.: O15379
• Gene Symbols: HDAC3, SMAP45, HD3, RPD3, RPD3-2
• Host Species: Mouse
• Immunogen: peptide (NEFYDGDHDNDKESDVEI) corresponding to amino acids 411-428 of human HDAC3
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003883
• Includes: This ChIPAb+ HDAC3 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Bovine, Hamster, Human, Mouse
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG2a

For Research Use Only