MilliporeSigma™ Upstate™ Histone H2A.Bbd, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-46MI	25 Assays	Unconjugated

Catalog No. 17-102-46MI Supplier MilliporeSigma™ Supplier No. 1710246

Description

Rabbit Polyclonal Antibody

Specifically detects Histone H2A.Bbd Clone: in Human, Mouse samples, and it is validated for ChIP, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blotting

Histone H2A.Bbd (Barr body-deficient), also known as histone H2A-Bbd type 1, is an atypical histone variant. This histone variant associates with H2B and replaces histone H2A in the nucleosome. In contrast to other H2A histones, this variant lacks conserved residues that are targets of post-translational modifications. Consequently this H2A.Bbd variant is about 15 amino acids shorter and only 48% similar to the canonical H2A. The major differences between the two are found in the docking domain that links H2A -H2B to the H3-H4 tetramer. Nucleosomes containing this histone adopt a more relaxed structure and protect ∽118 base pairs of DNA instead of the 147 base pairs found in classical nucleosomes. This variant histone is associated with active X chromosomes and autosomes, and is typically absent from inactive X chromosomes.

Specifications

• Antigen: Histone H2A.Bbd
• Applications: ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Histone H2A.Bbd (rabbit polyclonal). One vial containing 100μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Concentration: 0.7mg/mL . Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide.ChIP primers, PGK1. One vial containing 75μL of 5μM of each primer specific for human PGK1. FOR: CCT TCC CCC ACG CTC CTA; REV: TTG TGC AGG AAC CTG TGA GAT G
• Gene Accession No.: P0C5Y9
• Gene Symbols: H2A.Bbd, H2AFB1
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to human Histone H2A.Bbd.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001017990
• Test Specificity: This antibody recognizes human Histone H2A.Bbd.
• Includes: This ChIPAb+ Histone H2A.Bbd -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only