MilliporeSigma™ Upstate™ Histone H2A.Z, Sheep Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-100-48MI	25 Assays	Unconjugated

Catalog No. 17-100-48MI Supplier MilliporeSigma™ Supplier No. 1710048

Description

Sheep Polyclonal Antibody

Specifically detects Histone H2A.Z Clone: in Human samples, and it is validated for ChIP, Western Blotting

H2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

Specifications

• Antigen: Histone H2A.Z
• Applications: ChIP Assay, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Histone H2A.Z (Sheep polyclonal IgG). One vial containing 25μg of affinity purified sheep polyclonal IgG in PBS, pH 7.4 containing 1% BSA with 0.02% sodium azide, before the addition of 30% glycerol. Normal Sheep IgG. One vial containing 25μg of protein G purified sheep IgG in 0.02 M potassium phosphate, 0.15M NaCl, pH 7.2, with 0.01% sodium azide, before the addition of 30% glycerol. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: P0C0S5
• Gene Symbols: H2A.z, H2A/z , H2AZ, MGC117173, H2AFZ
• Host Species: Sheep
• Immunogen: Linear peptide corresponding the C-terminal amino acids 113-127 of human Histone H2A.Z.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_036544
• Test Specificity: Recognizes Histone H2A.Z, Mr ∽15kDa.
• Includes: This ChIPAb+ Histone H2A.Z -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only