MilliporeSigma™ Upstate™ Histone H3.3, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-45MI	25 Assays	Unconjugated

Catalog No. 17-102-45MI Supplier MilliporeSigma™ Supplier No. 1710245

Description

Rabbit Polyclonal Antibody

Specifically detects Histone H3.3 Clone: in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Dot Blot, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.

Specifications

• Antigen: Histone H3.3
• Applications: ChIP Assay, Dot Blot, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Histone H3.3 (rabbit polyclonal). One vial containing 50μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. Control Primers, GAPDH Coding Region D2 Human. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. FOR: GCC ATG TAG ACC CCT TGA AGA G; REV: ACT GGT TGA GCA CAG GGT ACT TTA T
• Gene Accession No.: P84243
• Gene Symbols: H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to human Histone H3.3.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_002098
• Test Specificity: This antibody recognizes human Histone H3.
• Includes: This ChIPAb+ Histone H3.3 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only