MilliporeSigma™ Upstate™ Histone H3 (CT), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-54MI	25 Assays	Unconjugated

Catalog No. 17-102-54MI Supplier MilliporeSigma™ Supplier No. 1710254

Description

Rabbit Polyclonal Antibody

Specifically detects Histone H3 (CT) Clone: in Chicken, Human, Mouse, Rat, S. cerevisiae, Yeast samples, and it is validated for ChIP, Dot Blot, Immunocytochemistry, Peptide Inhibition Assay, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: Histone H3 (CT)
• Applications: ChIP Assay, Dot Blot, Immunocytochemistry, Inhibition Assays, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Histone H3 (C-Term) (rabbit polyclonal). One vial containing 25μL of rabbit antiserum diluted 1:2 in storage buffer (0.02M Phosphate, 0.25M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%. Normal Rabbit Serum. One vial containing 25μL of antiserum containing 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
• Gene Accession No.: Q16695
• Gene Symbols: HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3T, H3/g, H3.4, H3/t
• Host Species: Rabbit
• Immunogen: KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
• Purification Method: Unpurified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_002107.3
• Test Specificity: Recognizes C-Terminal region of Histone H3, Mr 17kDa
• Includes: This ChIPAb+ Histone H3 (C-Term) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Chicken, Human, Mouse, Rat, S. cerevisiae, Yeast
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Serum

For Research Use Only