MilliporeSigma™ Upstate™ Histone H3 (CT)α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-100-46MI	25 Assays	Unconjugated

Catalog No. 17-100-46MI Supplier MilliporeSigma™ Supplier No. 1710046

Description

Rabbit Monoclonal Antibody

Specifically detects Histone H3 (CT)α Clone: in Chicken, Human, Mouse, Rat, S. cerevisiae, Yeast samples, and it is validated for ChIP, Dot Blot, Immunoprecipitation, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term Histone H3 alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Specifications

• Antigen: Histone H3 (CT)α
• Applications: ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-Histone H3 (C-term) (rabbit monoclonal IgG). One vial containing 100μL of cultured supernatant in 0.05% sodium azide. Negative Control Supernatant (rabbit). One vial containing 100μL of cultured supernatant in 0.05% sodium azide. ChIP Primers, β-Globin. One vial containing 75μL of 5μM of each primer specific for human β-Globin. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
• Gene Accession No.: Q16695
• Gene Symbols: H3.4, H3/g, H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
• Host Species: Rabbit
• Immunogen: KLH-conjugated, synthetic peptide corres-ponding to the C-terminus of human Histone H3.
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484
• Test Specificity: Recognizes Histone H3 near the C-terminus.
• Includes: This ChIPAb+ Histone H3 (C-term) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Chicken, Human, Mouse, Rat, S. cerevisiae, Yeast
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Culture Supernatant
• Isotype: IgG

For Research Use Only