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MilliporeSigma™ Upstate™ Histone H3 (Unmodified Lys4)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No. 17675MI Shop All MilliporeSigma Products
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Quantity:
25 Assays
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
17-675-MI 25 Assays Unconjugated
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Catalog No. 17-675-MI Supplier MilliporeSigma™ Supplier No. 17675
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Description

Mouse Monoclonal Antibody

Specifically detects Histone H3 (Unmodified Lys4)α Clone: in Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

TRUSTED_SUSTAINABILITY

Specifications

Antigen Histone H3 (Unmodified Lys4)α
Applications ChIP Assay, Western Blot
Classification Monoclonal
Conjugate Unconjugated
Formulation Anti-Histone H3 (Unmodified Lys4) (mouse monoclonal IgG2bκ, clone CMA301). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers β-Globin. One vial containing 75μL of 5μM of each primer specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
Gene Accession No. Q66I33
Gene Symbols HIST3H3; H3FT; H3/t; H3t; H3/g; H3.4; H3T
Host Species Mouse
Immunogen The histone H3 (Unmodified Lys4) purified antibody is made against a synthetic peptide corresponding to amino acids 1-12 of histone H3.
Purification Method Protein G purified
Quantity 25 Assays
Regulatory Status RUO
Research Discipline Epigenetics and Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NM_003493
Test Specificity Recognizes unmodified histone H3 (Lys4), Mr 17kDa. Epitope specificity of antibody as determined by ELISA maps near Lys4. Antibody tolerates modifications on Lys9 and Ser10 but not Lys4 or Thr31.
Includes This ChIPAb+ Histone H3 (Unmodified Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Target Species Vertebrates
Content And Storage -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Form Purified
Isotype IgG2b κ
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For Research Use Only

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