MilliporeSigma™ Upstate™ Histone H4α, Rabbit Monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-100-47MI	25 Assays	Unconjugated

Catalog No. 17-100-47MI Supplier MilliporeSigma™ Supplier No. 1710047

Description

Rabbit Monoclonal Antibody

Specifically detects Histone H4α Clone: in Human samples, and it is validated for ChIP, Western Blotting

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

Specifications

• Antigen: Histone H4α
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-Histone H4 (rabbit monoclonal IgG). One vial containing 50μL of protein A purified rabbit monoclonal IgG supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before addition of glycerol to 40%. Normal Rabbit IgG. One vial containing 75μg of normal rabbit IgG in 75μL storage buffer. ChIP Primers, β-Globin. One vial containing 75μL of 5μM of each primer specific for human β-Globin. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
• Gene Accession No.: P62805
• Gene Symbols: HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E
• Host Species: Rabbit
• Immunogen: KLH-conjugated synthetic peptide corres-ponding to amino acids 17-28 of Histone H4.
• Purification Method: Protein A purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics, Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001029249
• Test Specificity: Recognizes Histone H4.
• Includes: This ChIPAb+ Histone H4 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified

For Research Use Only