MilliporeSigma™ Human iPS Reprogramming Boost Supplement II

Description

contains three proprietary small molecules (TGF-β RI Kinase Inhibitor IV, Sodium Butyrate and PS48) and aids in somatic cell reprogramming by 10-15 fold.

EMD Millipore’s Human iPS Reprogramming Boost Supplement II contains three proprietary small molecules (TGF-β RI Kinase Inhibitor IV, Sodium Butyrate and PS48) in amounts sufficient to supplement 350mL of human ES cell maintenance medium. When used in conjunction with the Human STEMCCA lentivirus reprogramming kits (SCR544, SCR545, and SCR548), the Human iPS Reprogramming Boost Supplement II enhances the efficiency of human iPS colony formation by 10 – 15 fold and shortens the timescale to establish fully reprogrammed colonies by 50%. The colonies formed possess the distinctive flat 2D morphology that are reminiscent of normal human ES cell colonies and can be easily passaged. Fully reprogrammed human iPS colonies that are SSEA-4-positive, TRA-1-60-positive and Hoechst-Dim are readily established in approximately 20 – 25 days.

Specifications

• Content And Storage: TGF-β RI Kinase Inhibitor IV Supplement (1000X): (Mfr. No. CS210445) One (1) vial containing 400μL of the inhibitor in high quality DMSOPS48 Supplement (1000X): (Mfr. No. CS210447) One (1) vial containing 400μL of the inhibitor in high quality DMSO. Sodium Butyrate Supplement (1000X): (Mfr. No. CS210446) One (1) vial containing 400μL of the inhibitor in water. Store at -20°C
• For Use With (Application): Enhancing efficiency of human iPS colony formation by 10 to 15 fold
• Quantity: 1 Kit