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MilliporeSigma™ Human STEMCCA Constitutive Polycistronic Lentivirus Reprogramming Kit

Catalog No. SCR544 Shop All MilliporeSigma Products
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Description

contains high titer polycistronic lentivirus and Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

The Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The STEMCCA vector is comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.
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Specifications

Quantity 1 Kit
Content And Storage Human STEMCCA Constitutive (OKSM) Lentivirus: (Mfr. No. SCR544-1) Two (2) vials of EF1α-hSTEMCCA (OKSM) Lentivirus (Mfr. No. CS204502). Each vial contains 15μL of high titer lentivirus. Polybrene Transfection Reagent: (Mfr. No. TR-1003-50UL) One (1) vial containing 50μL of 10mg/mL stock> Store at -80°C
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