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MilliporeSigma™ Human STEMCCA Cre-Excisable Constitutive Polycistronic Lentivirus Reprogramming Kit

Catalog No. SCR545
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Quantity:
1 Kit

contains high titer polycistronic lentivirus and Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

The Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The Cre-excisable STEMCCA vector, comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM) separated by the self-cleaving 2A peptide and IRES sequences, includes flanking LoxP sites that enable Cre-mediated excision of the viral transgenes following reprogramming (Sommer CA, 2009).
TRUSTED_SUSTAINABILITY

Specifications

Quantity 1 Kit
Content And Storage Human STEMCCA Cre-Excisable Constitutive (OKSM) Lentivirus: (Mfr. No. SCR545-1) Two (2) vials of EF1α-hSTEMCCA-LoxP (OKSM) Lentivirus (Mfr. No. CS204503). Each vial contains 15μL of high titer lentivirus. Polybrene Transfection Reagent: (Mfr. No. TR-1003-50UL) One (1) vial containing 50μL of 10mg/mL stock. Store at -80°C
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