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MilliporeSigma™ Introductory pETBlue-1 Perfectly Blunt Cloning Kit

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70-633-3 100 Reactions
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Catalog No. 70-633-3 Supplier MilliporeSigma™ Supplier No. 706333
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Description

The Perfectly Blunt™ Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase. This approach enables the use of high-fidelity proofreading enzymes for amplification, thus decreasing the probability of generating mutations in the target sequence. In addition, under many conditions blunt cloning is more efficient than T-cloning, most likely due to the observation that the efficiency of single dA addition by Taq DNA polymerase varies significantly depending on the sequence context of the DNA ends, and even the number of PCR cycles performed.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at -70°C.
Quantity 100 Reactions

For Research Use Only

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