MilliporeSigma™ Upstate™ JMJD6, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-63MI	25 Assays	Unconjugated

Catalog No. 17-102-63MI Supplier MilliporeSigma™ Supplier No. 1710263

Description

Rabbit Polyclonal Antibody

Specifically detects JMJD6 Clone: in Human samples, and it is validated for ChIP, Western Blotting

JmjC domain-containing protein 6 (JMJD6) is a dioxygenase belonging to group of proteins known as the Jmjc-domain containing proteins. This family of proteins is a group of redox enzymes specifically involved in catalyzing oxidation reactions, and are characterized by the presence of a highly conserved jumonji C (Jmjc)-domain. JMJD6 can function both as a lysyl-hydroxylase and a histone arginine demethylase. As a lysyl-hydroxylase, JMJD6 catalyzes 5-hydroxylation of target protein lysine residues. As an arginine demethylase, JMJD6 has a critical part to play in histone code by demethylating of histone H3 and histone H4. It has also been shown to mediate U2AF2/U2AF65 5-hydrozylation, thereby functioning as a regulator for RNA splicing through affecting U2AF2/U2AF65 pre-mRNA activity. JMJD6 is a critical component of embryogenesis for the differentiation of various organs, and may act as a regulator for hematopoietic regulation and macrophage cytokine response. High expression of JMJD6 has been observed in heart, kidney and skeletal muscle. Medium to low expression has been seen in brain, liver, lung, ovary, pancreas, placenta, prostate, testis, thymus, and spleen.

Specifications

• Antigen: JMJD6
• Applications: ChIP Assay, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-JMJD6 (rabbit polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, human β-globin. One vial containing 75μL of each primer (5μM) specific for the human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC; REV: TTT ATG CCC AGC CCT GGC TC
• Gene Accession No.: Q6NYC1
• Gene Symbols: JMJD6, PTDSR1, PTDSR, PSR
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to human JMJD6.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001074930
• Test Specificity: This antibody recognizes JMJD6.
• Includes: This ChIPAb+ JMJD6 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only