MilliporeSigma™ Upstate™ LSF, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-52MI	25 Assays	Unconjugated

Catalog No. 17-102-52MI Supplier MilliporeSigma™ Supplier No. 1710252

Description

Rabbit Polyclonal Antibody

Specifically detects LSF Clone: in Human, Mouse samples, and it is validated for ChIP, Western Blotting

Transcription factor LSF (late simian virus 40 factor) also known as transcription factor LSF, CP2, LBP-1c, and SEF is expressed in a variety of cell types including brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. LSF is essential for cell cycle progression at the G1/S transition after reentry into the cell cycle from G0. LSF plays an important role in DNA synthesis and cell survival. A major cellular target of LSF is the thymidylate synthase (TS) gene coding for the rate limiting enzyme in the production of dTTP. Disruption of LSF function results in apoptosis at S phase or to cell cycle arrest at the G1/S transition.

Specifications

• Antigen: LSF
• Applications: ChIP Assay, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-LSF (rabbit polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Normal Rabbit IgG. One vial containing 125μg of Rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse switch (S) regions α. One vial containing 75μL of 5μM of each primer specific for Mouse switch (S) regions α. FOR: CTA GAC CGG GCA GAC CTA GA; REV: AAA GCC CAT TCC AGC CTA GT
• Gene Accession No.: Q12800
• Gene Symbols: TFCP2, LSF, SEF
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to amino acids 310-335 of human LSF.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005644
• Includes: This ChIPAb+ LSF -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only