MilliporeSigma™ Upstate™ Monomethyl-Histone H3 (Lys4)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-676-MI	25 Assays	Unconjugated

Catalog No. 17-676-MI Supplier MilliporeSigma™ Supplier No. 17676

Description

Mouse Monoclonal Antibody

Specifically detects Monomethyl-Histone H3 (Lys4)α Clone: in Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: Monomethyl-Histone H3 (Lys4)α
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-Monomethyl-HisPtone H3 (Lys4) (mouse monoclonal), One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, human GAPDH coding region, . One vial containing 75μL of 5μM each primer specific for human GAPDH coding region. FOR: GGC TCC CAC CTT TCT CAT CC; REV: GGC CAT CCA CAG TCT TCT GG
• Gene Accession No.: Q16695
• Gene Symbols: HIST3H3; H3FT; H3/t; H3t; MGC126888; H3/g; H3.4; H3T; MGC126886
• Host Species: Mouse
• Immunogen: Peptide corresponding to Histone H3 containing the sequence [ART(me1-K)QTARKSTGC] on which Lys4 is acetylated.
• Purification Method: Protein A purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003493
• Test Specificity: This antibody detects Monomethyl-Histone H3 methylated at Lys4.
• Includes: This ChIPAb+ Monomethyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Vertebrates
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only