MilliporeSigma™ Upstate™ Monomethyl-Histone H4 (Lys20), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-651-MI	25 Assays	Unconjugated

Catalog No. 17-651-MI Supplier MilliporeSigma™ Supplier No. 17651

Description

Rabbit Polyclonal Antibody

Specifically detects Monomethyl-Histone H4 (Lys20) Clone: in Human, Plant, Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Specifications

• Antigen: Monomethyl-Histone H4 (Lys20)
• Applications: ChIP Assay, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-monomethyl-Histone H4 (Lys20) (rabbit polyclonal IgG). One vial containing 100μg of affinity purified antibody in 100μL 0.1M Tris-Glycine (pH 7.4), 15mM NaCl, and 0.05% NaN₃. Normal Rabbit IgG. One vial containing 125μg of purified rabbit IgG in 125μL storage buffer. ChIP Primers GAPDH Coding Region. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region. FOR: GGC TCC CAC CTT TCT CAT CC; REV: GGC CAT CCA CAG TCT TCT GG
• Gene Accession No.: P62805
• Gene Symbols: H4/A , H4/B, H4/C , H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/J, H4/M, H4/N, H4/O , H4/a , H4F2, H4FA , H4FB , H4FC , H4FD, H4FE, H4FG, H4FH, H4FI , H4FJ, H4FK, H4FM , H4FN, H4FO, HIST2H4
• Host Species: Rabbit
• Immunogen: The immunogen was a synthetic peptide corresponding to amino acids 15-24 of histone H4, monomethylated on Lys20.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003538.3
• Test Specificity: Recognizes histone H4, Mr ∽11kDa.
• Includes: This ChIPAb+ Monomethyl-Histone H4 (Lys20) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Plant, Vertebrates
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only