MilliporeSigma™ Upstate™ NFκB p65 (RelA)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-100-60MI	25 Assays	Unconjugated

Catalog No. 17-100-60MI Supplier MilliporeSigma™ Supplier No. 1710060

Description

Mouse Monoclonal Antibody

Specifically detects NFκB p65 (RelA)α Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Western Blotting

The transcription factor NFkappaB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFkappaB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFkappaB1 (p50 and its precursor p105), and NFkappaB2 (p52 and its precursor p100). NFkappaB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFkappaB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFkappaB is the most abundant in cells.

Specifications

• Antigen: NFκB p65 (RelA)α
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-NFκB p65 (RelA) (mouse monoclonal). One vial containing 100μg of protein A purified monoclonal IgG3 in a total volume of 143μL in a buffer containing 0.02M phosphate buffer, 0.25M NaCl, pH 7.6 with 0.09% sodium azide, before the addition of glycerol to 30%. Nornal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, IĸBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
• Gene Accession No.: Q04206
• Gene Symbols: RELA, NFKB, p65, p50/p65
• Host Species: Mouse
• Immunogen: Peptide corresponding to human p65 coupled to BSA.
• Purification Method: Protein A purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_068810.3
• Test Specificity: This antibody recognizes an epitope overlapping the nuclear location signal (NLS) of the p65 subunit of the NFkB heterodimer. Thus it selectively binds to the activated form of NFkB.
• Includes: This ChIPAb+ NFκB p65 (RelA) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG3

For Research Use Only