MilliporeSigma™ Orexin A (14-33) (Ab-1), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ PC3451SET

Catalog No. PC3451SET

Description

Specifically detects Orexin A (14-33) (Ab-1) Clone: in Bovine, Human, Mouse, Rat samples, and it is validated for ELISA, Immunofluorescence, Immunohistochemistry (Frozen)

The hypothalamus has been shown to play a central role in the regulation of energy homeostasis and in the control of feeding behavior. Two homologous peptides have recently been identified for which the message and protein have been localized to the lateral hypothalamus. Orexin A (hypocretin 1), a 33 amino acid peptide, and orexin B (hypocretin 2), a 28 amino acid peptide, are both encoded by a single mRNA transcript. These peptides stimulate food consumption in a dose-dependent manner when administered intracerebroventricularly, and precursor mRNA levels are upregulated upon fasting. In addition to the orexins (hypocretins), two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides. Competitive radioligand binding assays suggest that the orexin receptor-1 is selective for orexin A, while orexin receptor-2 is nonselective for both peptides.

Specifications

• Antigen: Orexin A (14-33) (Ab-1)
• Classification: Polyclonal
• Formulation: 100μg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25μg lyophilized control peptide.
• Immunogen: a synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
• Regulatory Status: RUO
• Test Specificity: Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.
• Target Species: Bovine, Human, Mouse, Rat
• Form: Purified

• Applications: ELISA, Immunofluorescence, Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Rabbit
• Quantity: 1 Set
• Primary or Secondary: Primary
• Includes: 100μg antibody in 0.05M sodium phosphate buffer, 0.2% gelatin and 25μg lyophilized control peptide.
• Content And Storage: 2°C to 8°C
• Isotype: IgG

For Research Use Only