MilliporeSigma™ Upstate™ PCAF, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 1710532

Catalog No. 17-105-32MI

Description

Specifically detects PCAF Clone: in Human samples, and it is validated for ChIP, Western Blotting

P/CAF(sometimes called KAT2B) is a transcription cofactor with a histone acetyltransferase (HAT) activity. Consistent with this protein playing a direct role in transcriptional regulation, P/CAF can acetylate core histones H3 and H4, chromatin proteins HMG17 and HMG I(Y), as well as human immunodeficiency virus Tat, transcription factor MyoD, and general transcription factors TFIIE and TFIIF. A role for P/CAF in cell growth and differentiation is suggested by its interaction in vivo and in vitro with p300/CBP, two large nuclear proteins that bind to sequence-specific factors involved in cell growth/differentiation. A role for P/CAF in cell division is also suggested by data showing that this protein competes with E1A for binding sites in p300/CBP to counteract E1A's mitogenic activity.

Specifications

• Antigen: PCAF
• Classification: Polyclonal
• Formulation: Anti-PCAF (Rabbit Polyclonal). One vial containing 17.5μg purified rabbit polyclonal in 25μL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of 30% glycerol (0.7mg/mL final). Normal Rabbit IgG. One vial containing 125μg of purified Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, PRAME. One vial containing 75μL of 5μM of each primer specific for human preferentially expressed antigen in melanoma (chr22:22901678+22901804, hg19 build). FOR: GAA GCC TCG CCG GAA CTC; REV: TCC TCC CCA TCT CTG CAG AA
• Gene Symbols: GCN5, GCN5L, P/CAF, P, CAF, PCAF, KAT2B
• Immunogen: Recombinant protein corresponding to human PCAF (a.a. 782-832).
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes PCAF in regions between a.a. 782 to 832.
• Target Species: Human
• Form: Purified

• Applications: ChIP Assay, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: Q92831
• Host Species: Rabbit
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_003875
• Includes: This ChIPAb+ Validated Antibody and Primer Set conveniently includes the antibody, matched IgG negative control antibody and set control PCR primers that detect a known positive locus.
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only