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MilliporeSigma™ pET-44 Ek/LIC Vector Kit

Catalog No. 711443 Shop All MilliporeSigma Products
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pET-44 Ek/LIC Vector Kit
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Catalog No.
71-144-3 pET-44 Ek/LIC Vector Kit
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Catalog No. 71-144-3 Supplier MilliporeSigma™ Supplier No. 711443/DEL/DEL

Description

The pET-44 vectors incorporate the Nus·Tag™ technology, which allows target proteins to be expressed as fusions to the highly soluble NusA protein. According to a modeling study, the NusA protein has the highest potential for solubility among more than 4000 E. coli proteins, and therefore increases the proportion of soluble protein when expressed in E. coli as a fusion partner. Ek/LIC represents a highly flexible cloning strategy in which a single insert preparation can be simultaneously cloned into a wide variety of compatible Ligation Independent Cloning (LIC) vectors to allow high-throughput optimization of target protein expression.
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