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MilliporeSigma™ pET-47b(+) DNA Vector

Manufacturer:  MilliporeSigma™ 714613

Catalog No. 71-461-3

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The pET-47b(+) plasmid features the ability to express fusion proteins with an N-terminal His·Tag™ coding sequence that is cleavable with the human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S·Tag™ coding sequence is compatible with purification, detection and quantification.


Express fusion proteins with an N-terminal HisTag™ coding sequence that is cleavable with the human rhinovirus (HRV) 3C protease
10 μg
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