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MilliporeSigma™ pET-48b(+) DNA Vector

Catalog No. 714623 Shop All MilliporeSigma Products
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71-462-3 10 μg
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Catalog No. 71-462-3 Supplier MilliporeSigma™ Supplier No. 714623
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Description

The pET-48b(+) vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx·Tag™ thioredoxin protein that is cleavable with the human rhinovirus (HRV) 3C protease. Cloning sites are available for producing fusion proteins also containing a cleavable N- terminal His·Tag™ sequence for detection and purification. The plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the rhinovirus 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S·Tag™ coding sequence is compatible with purification, detection, and quantification.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at or below -70°C.
Quantity 10 μg

For Research Use Only

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