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MilliporeSigma™ pET-51b(+) DNA Vector

Catalog No. 715533 Shop All MilliporeSigma Products
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71-553-3 10 μg
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Catalog No. 71-553-3 Supplier MilliporeSigma™ Supplier No. 715533
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Description

The pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep·Tag™ II coding sequence that is cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His·Tag™ coding sequence with 10 histidines is compatible with purification, detection, and quantification.
TRUSTED_SUSTAINABILITY

Specifications

Promoter T7lac
Content And Storage Store at or below -70°C.
Quantity 10 μg
For Use With (Application) Cloning and high-level expression of target proteins fused with the 8 aa StrepTag™ II coding sequence that is cleavable with enterokinase (Ek) protease

For Research Use Only

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