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MilliporeSigma™ pET-52b(+) DNA Vector
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Quantity:
10 μg
Description
The pET-52b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep·Tag™ II coding sequence that is cleavable with human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LysGluValLysPheGl(down arrow)GlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. It also encodes an optional C-terminal thrombin protease cleavage site and His·Tag™ coding sequence with 10 histidines.
Specifications
Specifications
| Promoter | T7lac |
| Content And Storage | Store at or below -70°C. |
| Quantity | 10 μg |
| For Use With (Application) | Cloning and high-level expression of target proteins fused with the 8 aa StrepTag™ II coding sequence that is cleavable with human rhinovirus (HRV) 3C protease |
For Research Use Only
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