MilliporeSigma™ Upstate™ Phospho-CREB (Ser133), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 1710131

Catalog No. 17-101-31MI

Description

Specifically detects Phospho-CREB (Ser133) Clone: in Hamster, Human, Mouse, Rat samples, and it is validated for ChIP, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blotting

CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

Specifications

• Antigen: Phospho-CREB (Ser133)
• Classification: Polyclonal
• Formulation: Anti-phospho-CREB (Ser133) (rabbit polyclonal). One vial containing 100μg of affinity purified rabbit serum in 0.1M Tris-Glycine (pH 7.4) 0.15M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers cFos CRE. One vial containing 75μL of 5μM of each primer specific for the cFos CRE. FOR: GGC CCA CGA GAC CTC TGA GAC A; REV: GCC TTG GCG CGT GTC CTA ATC T
• Gene Symbols: CREB1, CREB, MGC9284
• Immunogen: KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
• Quantity: 25 Assays
• Research Discipline: Signaling, Epigenetics and Nuclear Function
• Gene ID (Entrez): NM_134442
• Includes: This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Isotype: IgG

• Applications: ChIP Assay, Electromobility Shift Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: P16220
• Host Species: Rabbit
• Purification Method: Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes p43 phosphorylated CREB. May also recognize p30 and p38kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
• Target Species: Hamster, Human, Mouse, Rat
• Form: Purified

For Research Use Only