MilliporeSigma™ Upstate™ Phospho-Histone H3 (Ser28), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-69MI	25 Assays	Unconjugated

Catalog No. 17-102-69MI Supplier MilliporeSigma™ Supplier No. 1710269

Description

Rabbit Polyclonal Antibody

Specifically detects Phospho-Histone H3 (Ser28) Clone: in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Dot Blot, Immunocytochemistry, Peptide Inhibition Assay, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: Phospho-Histone H3 (Ser28)
• Applications: ChIP Assay, Dot Blot, Immunocytochemistry, Inhibition Assays, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti- Phospho-Histone H3 (Ser28) (Rabbit Polyclonal). One vial containing 125μl of purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Normal Rabbit IgG. Two vials containing 75μL of normal rabbit IgG in buffer containing 0.01M PBS with 0.1% sodium azide. ChIP Primers, human GAPDH promoter. One vial containing 75μL of 5μM each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: Q16695
• Gene Symbols: HIST3H3, H3FT
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser28.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484.1
• Includes: This ChIPAb+ Phospho-Histone H3 (Ser28) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only