MilliporeSigma™ Procathepsin K, Human, Recombinant Protein, E. coli

Catalog No.	Quantity
34-200-110UG	10 μg

Catalog No. 34-200-110UG Supplier MilliporeSigma™ Supplier No. 34200110UG

Description

Recombinant, human procathepsin K (amino acids 19-329) (GenBank target symbol = S79895, ACC No. P43235) expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site. Cathepsin K, a member of the papain superfamily of cysteine proteinases, plays an important role in osteoclast-mediated bone resorption and collagen degradation. Cathepsin K is synthesized as an inactive proenzyme that is converted to its mature, active form by proteolytic cleavage of the 99 amino acid propeptide domain. Inhibitors of cathepsin K include leupeptin (IC₅₀ = 70nM), E-64 (IC₅₀ = 5nM), and cystatin. Requires activation prior to use.

Specifications

• Concentration: Concentration on Label
• Formulation: In 500mM NaCl, 25mM Tris, pH 8.0.
• Purification Method: SDS-PAGE
• Quantity: 10 μg
• Source: E. coli
• Immunogen: Procathepsin K
• Storage Requirements: Following initial thaw, aliquot and freeze (−70°C). Following activation the enzyme is unstable and should include MMTS for storage (see recommended reaction conditions for activation).
• Regulatory Status: RUO
• Species: Human
• Recombinant: Recombinant
• Form: Liquid
• Purity or Quality Grade: ≥95%