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MilliporeSigma™ Protein Phosphatase, Lambda, Recombinant Protein, E. coli

Catalog No. 53951420KU Shop All MilliporeSigma Products
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20 kU
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53-951-420KU 20 kU
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Catalog No. 53-951-420KU Supplier MilliporeSigma™ Supplier No. 53951420KU
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Description

Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn2+-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.
TRUSTED_SUSTAINABILITY

Specifications

Formulation In 100mM NaCl, 50mM HEPES, 2mM DTT, 0.1mM MnCl2, 0.1mM EGTA, 50% glycerol, 0.01% BRIJ™ 35 Detergent, pH 7.5.
Preparation Method Further dilute in 50mM Tris-HCl, pH 7.8, 5mM DTT, 2mM MnCl2, and 100μg/mL BSA.
Purification Method SDS-PAGE
Quantity 20 kU
Source E. coli
Immunogen Protein Phosphatase, Lambda
Storage Requirements Following initial thaw, aliquot and freeze (−70°C).
Regulatory Status RUO
Recombinant Recombinant
Form Liquid
Purity or Quality Grade ≥95%
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