MilliporeSigma™ Upstate™ RNA Pol IIα, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-620-MI	25 Assays	Unconjugated

Catalog No. 17-620-MI Supplier MilliporeSigma™ Supplier No. 17620

Description

Mouse Monoclonal Antibody

Specifically detects RNA Pol IIα Clone: in Human, Mouse, Rat, S. cerevisiae, Yeast samples, and it is validated for ChIP, Immunofluorescence, Immunoprecipitation

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome

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Specifications

• Antigen: RNA Pol IIα
• Applications: ChIP Assay, Immunofluorescence, Immunoprecipitation
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-RNA Pol II (mouse monoclonal IgG1,purified). One vial containing 25μg of purified antibody in 25μL volume. Normal Mouse IgG. One vial containing 25μg of mouse IgG in 25μL volume. Control Primers p21. One vial containing 75μL of 5μM of each primer specific for a region of the human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: P24928
• Host Species: Mouse
• Immunogen: The RNA Pol II antibody is made against a peptide corresponding to the C-terminal domain of RNA Pol II.
• Purification Method: Protein G purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000928
• Test Specificity: Recognizes RNA Pol II
• Includes: RNA Pol II ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of RNA Pol II.
• Target Species: Human, Mouse, Rat, S. cerevisiae, Yeast
• Content And Storage: −20°C for one year from date of shipment
• Form: Purified
• Isotype: IgG1

For Research Use Only