MilliporeSigma™ Upstate™ SOX-2α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-56MI	25 Assays	Unconjugated

Catalog No. 17-102-56MI Supplier MilliporeSigma™ Supplier No. 1710256

Description

Mouse Monoclonal Antibody

Specifically detects SOX-2α Clone: in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Immunocytochemistry, Western Blotting

SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution. Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain a pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.

Specifications

• Antigen: SOX-2α
• Applications: ChIP Assay, Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-SOX-2 (mouse monoclonal). One vial containing 75μg of protein G purified monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 150mM NaCl, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.7mg/mL. Normal Mouse IgG. One vial containing 125μg purified mouse IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, nanog promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human nanog. FOR: GTT CTG TTG CTC GGT TTT CT; REV: TCC CGT CTA CCA GTC TCA CC
• Gene Accession No.: P48431
• Gene Symbols: SOX2
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to human SOX-2.
• Purification Method: Protein G purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Stem Cell Research
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003097
• Includes: This ChIPAb+ SOX-2 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only