MilliporeSigma™ Upstate™ TCF-4α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-101-09MI	25 Assays	Unconjugated

Catalog No. 17-101-09MI Supplier MilliporeSigma™ Supplier No. 1710109

Description

Mouse Monoclonal Antibody

Specifically detects TCF-4α Clone: in Human, Mouse samples, and it is validated for Chromatin Immunoprecipitation, Dot Blot, Western Blotting

TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

Specifications

• Antigen: TCF-4α
• Applications: ChIP Assay, Dot Blot, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-TCF-4 (mouse monoclonal). One vial containing 50μg of purified mouse monoclonal IgG2a in 50μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Normal Mouse IgG. Two vials containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, SP5 promote. One vial containing 75μL of 5μM of each primer specific for human SP5 promotor. FOR: GGG TCT CCA GGC GGC AAG; REV: AGC GAA AGC AAA TCC TTT GAA TCC
• Gene Accession No.: Q9NQB0
• Gene Symbols: TCF4, TCF-4, TCF7L2
• Host Species: Mouse
• Immunogen: Amino acids 31-331 of human TCF-4.
• Purification Method: Protein G chromatography
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): CAB97213.1
• Test Specificity: This antibody recognizes TCF-4
• Includes: This ChIPAb+ TCF-4 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG2a

For Research Use Only