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MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys27), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No. 17622MI Shop All MilliporeSigma Products
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Quantity:
25 Assays
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
17-622-MI 25 Assays Unconjugated
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Catalog No. 17-622-MI Supplier MilliporeSigma™ Supplier No. 17622
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Description

Rabbit Polyclonal Antibody

Specifically detects Trimethyl-Histone H3 (Lys27) Clone: in Human, Mouse samples, and it is validated for ChIP, Western Blotting

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Trimethyl-Histone H3 (Lys27)
Applications ChIP Assay, Western Blot
Classification Polyclonal
Conjugate Unconjugated
Formulation Anti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100μg protein A purified IgG in 100μL buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg purified Rabbit IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers human alpha-Satellite. One vial containing 75μL of 5μM of each control primer specific for human alpha-Satellite. FOR: CTG CAC TAC CTG AAG AGG AC; REV: GAT GGT TCA ACA CTC TTA CA
Gene Accession No. Q16695
Gene Symbols H3F3A, MGC87783, H3.3A, MGC87782, H3F3, H3.3B, H3F3B
Host Species Rabbit
Immunogen KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.
Quantity 25 Assays
Regulatory Status RUO
Research Discipline Epigenetics and Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NP_003484
Test Specificity trimethyl-Histone H3 (Lys27)
Includes Trimethyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Me3.
Target Species Human, Mouse
Content And Storage −20°C for one year
Form Purified
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For Research Use Only

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