MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys36), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-104-93MI	25 Assays	Unconjugated

Catalog No. 17-104-93MI Supplier MilliporeSigma™ Supplier No. 1710493

Description

Rabbit Polyclonal Antibody

Specifically detects Trimethyl-Histone H3 (Lys36) Clone: in Human, Mouse samples, and it is validated for ChIP-seq, Chromatin Immunoprecipitation, Dot Blot, Western Blotting

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the histone code hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation is necessary for efficient RNA polymerase II elongation and processivity.

Specifications

• Antigen: Trimethyl-Histone H3 (Lys36)
• Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Dot Blot, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Trimethyl Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 50μL (0.05mg/mL) purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 7.5μg Rabbit IgG in 75μL storage buffer containing 0.05% sodium azide. ChIP Primers, GAPDH coding D2. One vial containing 75μL of 5μM of each primer specific for human GAPDH coding region (chr12:6647453+6647539 hg19 build). FOR: 5’ GCC ATG TAG ACC CCT TGA AGA G 3’; REV: 5’ ACT GGT TGA GCA CAG GGT ACT TTA T 3’
• Gene Accession No.: Q16695
• Gene Symbols: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to human Histone H3 trimethylated at Lys36 with peptide sequence APATGGV(K)KPHRYRPGC
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484
• Test Specificity: Recognizes Trimethyl-Histone H3 (Lys36), Mr ∽17kDa.
• Includes: This ChIPAb+ Trimethyl-Histone H3 (Lys36) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Mouse
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified

For Research Use Only