MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys79), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-101-30MI	25 Assays	Unconjugated

Catalog No. 17-101-30MI Supplier MilliporeSigma™ Supplier No. 1710130

Description

Rabbit Polyclonal Antibody

Specifically detects Trimethyl-Histone H3 (Lys79) Clone: in Human, Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Multiplexing, Western Blotting

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.

Specifications

• Antigen: Trimethyl-Histone H3 (Lys79)
• Applications: Multiplex, Immunoassay, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-Trimethyl-Histone H3 (Lys79) (rabbit polyclonal). One vial containing 25μg of purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide before the addition of 30% glycerol. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for human GAPDH. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
• Gene Accession No.: Q16695
• Gene Symbols: HIST3H3, H3FT, H3t, H3T, H3/g, H3.4, H3/t
• Host Species: Rabbit
• Immunogen: Linear Peptide corresponding to human Histone H3 methylated at Lys79.
• Purification Method: Affinity Purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484.1
• Test Specificity: This antibody recognizes Histone H3 Trimethylated on Lys79.
• Includes: This ChIPAb+ Trimethyl-Histone H3 (Lys79) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Vertebrates
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only