MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys9)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-102-42MI	25 Assays	Unconjugated

Catalog No. 17-102-42MI Supplier MilliporeSigma™ Supplier No. 1710242

Description

Mouse Monoclonal Antibody

Specifically detects Trimethyl-Histone H3 (Lys9)α Clone: in Human, Vertebrate samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.

Specifications

• Antigen: Trimethyl-Histone H3 (Lys9)α
• Applications: ChIP Assay, Western Blot
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Anti-Trimethyl-Histone H3 (Lys9) (mouse monoclonal). One vial containing 25μL of purified mouse monoclonal IgG1 in PBS with 0.05% sodium azide before the addition of glycerol to 30%. Normal Mouse IgG. One vial containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. Control Primers, ZNF554. One vial containing 75μL of 5μM of each primer specific for the 3’ end of human ZNF554. FOR: CGG GGA AAA GCC CTA TAA AT; REV: TCC ACA TTC ACT GCA TTC GT
• Gene Accession No.: Q16695
• Gene Symbols: H3., H3/g, H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945
• Host Species: Mouse
• Immunogen: KLH-conjugated synthetic peptide corresponding to human Histone H3 trimethylated on Lys9.
• Purification Method: Protein G purified
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NM_003493
• Test Specificity: This antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is >27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.
• Includes: This ChIPAb+ Trimethyl-Histone H3 (Lys9) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Target Species: Human, Vertebrates
• Content And Storage: 2°C to 8°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at -20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only