MilliporeSigma™ Upstate™ Trimethyl-Histone H3 (Lys9), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Catalog No.	Quantity	Conjugate
17-625-MI	25 Assays	Unconjugated

Catalog No. 17-625-MI Supplier MilliporeSigma™ Supplier No. 17625

Description

Rabbit Polyclonal Antibody

Specifically detects Trimethyl-Histone H3 (Lys9) Clone: in Vertebrate samples, and it is validated for ChIP, Dot Blot, Western Blotting

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Specifications

• Antigen: Trimethyl-Histone H3 (Lys9)
• Applications: ChIP Assay, Dot Blot, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Anti-trimethyl-Histone H3 (Lys9) (rabbit polyclonal IgG). One vial containing 100μg protein A purified IgG in 100μL of 0.02 M Phosphate buffer, pH 7.4, 0.25 M NaCl, 0.05% sodium azide with 30% glycerol. Normal Rabbit IgG. One vial containing 125μg of normal rabbit IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, ZNF554. One vial containing 75μL of 5μM of each primer specific for ZNF554. FOR: CGG GGA AAA GCC CTA TAA AT; REV: TCC ACA TTC ACT GCA TTC GT
• Gene Accession No.: Q16695
• Gene Symbols: H3F3A, MGC87783, H3.3A, MGC87782, H3F3, H3.3B, H3F3B
• Host Species: Rabbit
• Immunogen: The trimethyl-histone H3 (Lys9) purified antibody is made against BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl lysine 9 of human Histone H3.
• Quantity: 25 Assays
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003484
• Test Specificity: Recognizes histone H3, Mr 17kDa, trimethylated at lysine 9.
• Includes: Trimethyl-Histone H3 (Lys9) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K9Me3.
• Target Species: Vertebrates
• Content And Storage: −20°C in undiluted aliquots for up to 12 months from date of receipt; Avoid repeated freeze/thaw cycles
• Form: Purified

For Research Use Only